Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
Author(s) -
Nicola Portolano,
Peter J. Watson,
Louise Fairall,
Christopher J. Millard,
Charles P. Milano,
Yun S. Song,
Shaun M. Cowley,
John W. R. Schwabe
Publication year - 2014
Publication title -
journal of visualized experiments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 91
ISSN - 1940-087X
DOI - 10.3791/51897-v
Subject(s) - hek 293 cells , recombinant dna , microbiology and biotechnology , biology , structural biology , synthetic biology , protein subunit , protein expression , embryonic stem cell , function (biology) , protein folding , cell culture , flag tag , computational biology , receptor , biochemistry , genetics , gene , fusion protein
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