Open Access<em>In Vitro</em> Reconstitution of Light-harvesting Complexes of Plants and Green Algae
Author(s) -
Alberto Natali,
Laura M. Roy,
Roberta Croce
Publication year - 2014
Publication title -
journal of visualized experiments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 91
ISSN - 1940-087X
DOI - 10.3791/51852
Subject(s) - thylakoid , in vitro , chloroplast , pigment , biophysics , recombinant dna , biochemistry , algae , biology , light harvesting complex , carotenoid , chemistry , botany , microbiology and biotechnology , photosynthesis , photosystem ii , gene , organic chemistry
In plants and green algae, light is captured by the light-harvesting complexes (LHCs), a family of integral membrane proteins that coordinate chlorophylls and carotenoids. In vivo, these proteins are folded with pigments to form complexes which are inserted in the thylakoid membrane of the chloroplast. The high similarity in the chemical and physical properties of the members of the family, together with the fact that they can easily lose pigments during isolation, makes their purification in a native state challenging. An alternative approach to obtain homogeneous preparations of LHCs was developed by Plumley and Schmidt in 198
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