Open AccessIsolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
Author(s) -
Kerstin Menck,
Daniel Behme,
Mathias Pantke,
Norbert Reiling,
Claudia R. Binder,
Tobias Pukrop,
Florian Klemm
Publication year - 2014
Publication title -
journal of visualized experiments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.596
H-Index - 91
ISSN - 1940-087X
DOI - 10.3791/51554-v
Subject(s) - buffy coat , centrifugation , isolation (microbiology) , macrophage , differential centrifugation , cell culture , monocyte , microbiology and biotechnology , immunology , biology , in vitro , biochemistry , genetics
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