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INT RODUCT ION Although supercritical fluid chromatography (SFC) is not a new technique, preparative SFC is becoming increasingly more popular with advances in instrumentation, software and chemistry. Basic principles for isolating compounds with SFC are similar to the fundamental rules for large-scale preparative liquid chromatography. In this study, we illustrate the effect of flow rate, sample dissolution solvent, and column selectivity on the isolation of compounds from a mixture. Columns packed with Optimum Bed Density (OBD) Technology1 and with matching chemistry ensure easy and predictable scalability from the small scale to larger preparative separations.

Basic Principles for Purification Using Supercritical Fluid Chromatography