A new method development and validation of dual wavelength UV Spectro Photometric method for Simultaneous estimation of Atenolol and Amlodipine besylate in combined dosage form

Atenolol(ATN)is chemically 4‐(2‐hydroxy‐3‐isopropyl aminopropoxy)‐phenyl acetamide, is beta‐blocker seem to be equally effective as an antihypertensive, anti‐anginal and antiarrhymthmic drug. It is widely used cardiovascular drug in combination with Amlodipine. Amlodipine Besylate (AMN) is chemically3‐ethyl‐5‐methyl‐(4RS)‐2‐[(2‐aminoethoxy) methyl]‐4‐(2‐chlorophenyl)‐6‐methyl‐1,4‐dihydropyrid ine‐3,5‐dicarboxylatebenzene sulphonate, is calcium channel blocker used as potent coronary and peripheral vasodilator and in bradycardia. Literature survey reveals that various analytical methods have been reported for the assay of atenolol and amlodipine besylate in pure form and in pharmaceutical formulations. Non aqueous titration method is specified in Indian Pharmacopoeia for the assay of atenolol. While British Pharmacopoeia described liquid chromatography method for the assay of amlodipine besylate. Other methods such as derivative spectroscopy2,3, simultaneous spectroscopic estimation4,5,HPLC6‐11,RP‐HPLC12,13,Colourimetr y, gas chromatography, difference spectroscopy14, HP‐TLC15 were reported for the estimation of atenolol and amlodipine in individual formulations and Journal of Comprehensive Pharmacy