Open AccessConstruction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells
Author(s) -
Hongbo Shi,
Jinli Lou,
Honglin Shi,
Feng Ren,
Yu Chen,
Zhongping Duan
Publication year - 2017
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v23.i2.224
Subject(s) - bacterial artificial chromosome , recombinant dna , electroporation , microbiology and biotechnology , restriction enzyme , kanamycin , biology , homologous recombination , bamhi , escherichia coli , shuttle vector , restriction digest , gene , genetics , vector (molecular biology) , genome
To prepare a Gpm6a/Reelin GFPCreERT2 construct with a rapid and reliable strategy using a bacterial artificial chromosome (BAC).
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