Open AccessGenomic DNA extraction from hair sacs of pigs using modified phe-nol-chloroform method
Author(s) -
Jiying Wang,
Ying Yu,
Lixia Feng,
Huaizhong Wang,
Qin Zhang
Publication year - 2010
Publication title -
hereditas (beijing)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.125
H-Index - 16
ISSN - 0253-9772
DOI - 10.3724/sp.j.1005.2010.00752
Subject(s) - genomic dna , chloroform , dna , extraction (chemistry) , dna extraction , biology , chromatography , phenol , chemistry , microbiology and biotechnology , biochemistry , polymerase chain reaction , gene , organic chemistry
In referring to various methods for genomic DNA extraction from different tissues, we modified the classical phenol-chloroform procedure and reaction system for use in genomic DNA extraction from pig hair sacs. With the modified the phenol-chloroform method we successfully obtained high quality genomic DNA from pig hair sacs. Genomic DNA can be extracted from sacs of one to six pig hairs with satisfied quantity and quality for the need of PCR-based molecular ex-periment.
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