Open AccessA reliable and affordable 3D tumor spheroid model for natural product drug discovery: A case study of curcumin
Author(s) -
Loh TengHern Tan,
Liang Ee Low,
Siah Ying Tang,
Wei Hsum Yap,
LayHong Chuah,
Chim Kei Chan,
LearnHan Lee,
Bey Hing Goh
Publication year - 2019
Publication title -
progress in drug discovery and biomedical science
Language(s) - English
Resource type - Journals
ISSN - 2710-6039
DOI - 10.36877/pddbs.a0000017
Subject(s) - spheroid , drug discovery , curcumin , in vivo , natural product , cytotoxicity , computer science , computational biology , 3d model , anticancer drug , in vitro , drug , drug development , biochemical engineering , biomedical engineering , bioinformatics , biology , pharmacology , artificial intelligence , engineering , microbiology and biotechnology , biochemistry
Three-dimensional cell culture methods revolutionize the field of anticancer drug discovery, forming an important link-bridge between conventional in vitro and in vivo models and conferring significant clinical and biological relevant data. The current work presents an affordable yet reproducible method of generating homogenous 3D tumor spheroids. Also, a new open source software is adapted to perform an automated image analysis of 3D tumor spheroids and subsequently generate a list of morphological parameters of which could be utilized to determine the response of these spheroids toward treatments. Our data showed that this work could serve as a reliable 3D cell culture platform for preclinical cytotoxicity testing of natural products prior to the expensive and time-consuming animal models
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