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Background:  Staphylococcus aureus and Escherichia coli are infection agents. The onion is known to have antibacterial properties. Meanwhile, sugar paste is effective to inhibit bacterial growth.   Objective:  This study aimed to identify the antibacterial activity of onion extract (Allium cepa Linn) and sugar paste mixture on staphylococcus aureus and Escherichia coli by in vitro.   Methods:  We used a post-test-only control group design with a completely random design. The onion extract with sugar paste mixture was divided into 4 concentrations; 25%, 50%, 75% and 100% with 3 repetitions. The data were analyzed by measuring inhibition zone diameter and tested using the One-Way Analysis of Variance (ANOVA) and Tukey test.   Results:  Findings showed that on average, the antibacterial activity of onion extract and sugar paste mixture on staphylococcus aureus was 14.57mm, 17.44mm, 18.36mm, and 22.28mm, respectively 25%, 50%, 75%, and 100% concentrations. Meanwhile, on Escherichia coli, it was 17.27mm, 19.67mm, 20.31mm, and 21.62mm.   Conclusion:  onion extract and sugar paste mixture can inhibit the growth of Staphylococcus aureus and Escherichia coli.

INHIBITORY EFFECT OF ONION (ALLIUM CEPA LINN) AND SUGAR PASTE MIXTURE ON STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI BY IN VITRO