IDENTIFIKASI DAN STUDI POLA FRAGMENTASI JAMU TERKONFIRMASI FENILBUTAZON MENGGUNAKAN LIQUID CHROMATOGRAPHY MASS SPECTROSCOPY (LCMS)

The Jamu is one type of traditional herbal medicine that still popular among the community until now. In twenty years  often found the jamu containing synthetic drugs. Drug chemical which is commonly added to this traditional herbal  medicine  include  the  class  of  drugs  of  NAISD’s  namely  phenylbutazone. This  study  was  conducted  to  determine  the  possibility  of  presence  of phenylbutazone in the Jamu. The beginning of this study by initial screening using Thin Layer Chromatography (TLC) method. The results showed that 4 of the 6 samples of the positive contain phenylbutazone based TLC method.  These 4 samples were then analyzed using the Liquid Chromatography Mass Spectroscopy (LCMS) method. The stationary phase is Octadecyl Silica (C18) and the Mobile Phase are ultrapure water motion: acetonitrile (90 : 10% v/v) with isocratic elution system and positive ion mode. Based on LCMS chromatogram data, the retention time (Rf) of phenylbutazone is 1.89 minutes. The retention time of samples same with  the retention time of phenylbutazone. The next approach, based on the fragmentation pattern of Mass Spectroscopy. Based on mass spectrum data, there are 2 formed fragmentation paths. The first pattern is the fragmentation process of phenylbutazone (309 m / z), forming 2-butylmalonaldehyde (129 m / z) and 1,2-diphenylhydrazine (184 m / z).  The second fragmentation pattern was obtained by the Indol 3-H pathway, producing 3-butylidene-3H-indol-2-olate fragments (188 m / z) and 3H-indole-2,3-diide (114 m / z). Based on the analysis using the LCMS method, four samples containing phenylbutazone, Samples A, C, D and E. The four samples should not be allowed to be traded and consumed for  violating  the  Regulation  of  the  Minister  of  Health  of  the  Republic  of  Indonesia  No  006  of  2012chapter  33  and  chapter  37  on  the  industry  and  traditional  medicine  business  that  traditional  medicine  is  prohibited  to contain chemicals from the isolation or synthetic drug efficacious.