DOCKING MOLEKULER SENYAWA B-KAROTEN DALAM TANAMAN KELOR (Moringa Oleifera L.) SEBAGAI PENGHAMBAT ENZIM TIROSINASE DENGAN AUTODOCK – VINA

Hydroquinone has been used in cosmetics because of its whitening activity. In previous studies, β-carotene in Moringa plants was also known as an inhibitor of the tyrosinase enzyme. It is necessary to know how the interaction mechanism of β-carotene with tyrosinase (5M8N) and which compounds between hydroquinone and β-carotene provide computationally better activity as whitening. Tyrosinase was prepared using Discovery Studio Visualizer. Ligands were prepared using Autodock 4.2. Autodock-Vina is used for ligand docking between proteins. The result is the binding affinity (kcal/mol) of the ligand to protein. Visualization of docking between ligands and proteins using the Ligplot + Program with a 1 year license. Media used for the docking process is a computer with an Intel Core i7-3770 CPU with a speed of 3.40 GHz 8 cores, 1920x1080p resolution, Jurnal Insan Farmasi Indonesia, 3(2) Desember 2020 (230-240) Bayu Herdi Al Huda p-ISSN 2621-3184 ; e-ISSN 2621-4032 doi: 10.36387/jifi.v3i2.540 231 VGA NVIDIA GeForce GTX 750, 8 GB RAM, Windows 8 64-bit. The docking results showed that the binding affinity of β-carotene to tyrosinase was -11.2 while hydroquinone with tyrosinase was -5.4 with RMSD 0. The results of visualization showed that β-carotene binds more amino acid receptors than hydroquinone. βcarotene in moringa has been shown to be active not only in wet laboratories, but also in dry laboratories.