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The main objective of the article was to understand undergraduate and distance learning students’ perception of Biological Sciences Course, at the Federal University of Sergipe (FUS), about the use of Digital Mobile Information and Communication Technologies (DMICT) while technological and pedagogical knowledge developed their initial training process. To achieve this goal, we also sought to know the profile related to the digital culture of the undergraduate students and to investigate how this public felt about the teachers’ digital literacy. It corresponded to a qualitative research with an exploratory bias, in which three researchers participated in the process of validating a questionnaire, and a case study was carried out with 33 (thirty-three) academics, distributed in 11 (eleven) personally and 22 (twenty-two) in Distance Education (DE), the majority being from the last period of undergraduate courses. It was found that all participants agreed that DMICT contributed to the students' learning, as long as they are used properly inside and outside the classroom. Most participants stated that they had satisfactory and advanced levels of knowledge and skills on the use of these technologies in their daily lives, thus pointing out an interconnection of this audience with cyberspace. However, 27.3% of the participants reported the absence of technological approaches during their training due to a number of factors, among them, the lack of interest from university professors and tutors, as well as demotivation of the class and lack of didactic and technological resources.Keywords: Digital Culture. Teacher training. Digital literacy.

As tecnologias móveis digitais da informação e comunicação presentes na formação inicial docente em ciências biológicas