Avaliação in vitro de quimioterápicos e fitoterápicos no controle de argulus sp

The present study aimed to evaluate the in vitro efficacy of the use of chemotherapeutic and phytotherapeutic agents in the control of Argulus sp. The experiment was carried out at the Carlos Eduardo Matiazze Fish Farming Base and at the Animal Production Laboratory (LAPA) of the Federal University of Rondônia (UNIR), the campus Presidente Médici. We analyzed 480 individuals of Argulus sp. collected in cultivation environment, being tested 15 treatments (1 control, 8 herbal products and 6 chemotherapeutic products). Each treatment contained three replicates, with 10 parasites/plate, containing 10 ml of solution prepared with the products at a concentration of 100 mg/L. The antiparasitic efficacy of the products was analyzed every 15 minutes by counting dead parasites. The tests lasted 180 min. In this in vitro assay, the results were satisfactory by testing the inhibitory effect of four chemotherapeutic and two herbal treatments on Argulus sp., where potassium permanganate, Neguvon® and formaldehyde solutions were 100% effective in the first fifteen minutes of experiment. And, lime, nem extract and copaíba showed 100%, 100% and 80%, respectively, at one hundred and eighty minutes, the final experimental evaluation time. The other products were able to reach the effectiveness of 30%, 26.7%, 26.7%, 13.3%, 6.7%, 3.3%, 0% and 0% for papaya, garlic, mast, ginger and albendazole, aloe vera, salt and clove, respectively. These results show that natural products are feasible, economically viable and environmentally friendly for fish farming, contributing to sustainability by reducing the use of chemicals, such as in fish breeding stations, for breeding and breeding control. Substances of plant origin can be presented as an alternative source for direct use or in the development of phytotherapic anti-parasites, requiring advance in studies for in vivo treatment, replacing chemical ones with natural ones. The results found also allow a greater basis for the control of the parasite Argulus sp., leading us to the development of new research, both in vitro and in vivo, leading to their diffusion and, in the future, to their application in cultivation environments.