Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study | Zendy

Daniela Martini | Zendy; Raúl DomínguezPerles | Zendy; Alice Rosi | Zendy; Michele Tassotti | Zendy; Donato Angelino | Zendy; Sónia Medina | Zendy; Cristian Ricci | Zendy; Alexandre Guy | Zendy; Camille Oger | Zendy; Letizia Gigliotti | Zendy; Thierry Durand | Zendy; Mirko Marino | Zendy; Hans Gottfried-Genieser | Zendy; Marisa Porrini | Zendy; Monica Antonini | Zendy; Alessandra Dei | Zendy; Riccardo C. Bonadonna | Zendy; Federico Ferreres | Zendy; Francesca Scazzina | Zendy; Furio Brighenti | Zendy; Patrizia Riso | Zendy; Cristian Del Bo’ | Zendy; Pedro Mena | Zendy; Ángel GilIzquierdo | Zendy; Daniele Del Rio | Zendy

Open Access

Effect of Coffee and Cocoa-Based Confectionery Containing Coffee on Markers of DNA Damage and Lipid Peroxidation Products: Results from a Human Intervention Study

Author(s) -

Daniela Martini,

Raúl DomínguezPerles,

Alice Rosi,

Michele Tassotti,

Donato Angelino,

Sónia Medina,

Cristian Ricci,

Alexandre Guy,

Camille Oger,

Letizia Gigliotti,

Thierry Durand,

Mirko Marino,

Hans Gottfried-Genieser,

Marisa Porrini,

Monica Antonini,

Alessandra Dei,

Riccardo C. Bonadonna,

Federico Ferreres,

Francesca Scazzina,

Furio Brighenti,

Patrizia Riso,

Cristian Del Bo’,

Pedro Mena,

Ángel GilIzquierdo,

Daniele Del Rio

Publication year - 2021

Publication title -

nutrients

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.418

H-Index - 115

ISSN - 2072-6643

DOI - 10.3390/nu13072399

Subject(s) - lipid peroxidation , guanosine , dna damage , oxidative stress , crossover study , food science , guanine , dose , chemistry , dna , comet assay , biochemistry , pharmacology , biology , medicine , gene , placebo , nucleotide , alternative medicine , pathology

The effect of coffee and cocoa on oxidative damage to macromolecules has been investigated in several studies, often with controversial results. This study aimed to investigate the effect of one-month consumption of different doses of coffee or cocoa-based products containing coffee on markers of DNA damage and lipid peroxidation in young healthy volunteers. Twenty-one volunteers were randomly assigned into a three-arm, crossover, randomized trial. Subjects were assigned to consume one of the three following treatments: one cup of espresso coffee/day (1C), three cups of espresso coffee/day (3C), and one cup of espresso coffee plus two cocoa-based products containing coffee (PC) twice per day for 1 month. At the end of each treatment, blood samples were collected for the analysis of endogenous and H 2 O 2 -induced DNA damage and DNA oxidation catabolites, while urines were used for the analysis of oxylipins. On the whole, four DNA catabolites (cyclic guanosine monophosphate (cGMP), 8-OH-2′-deoxy-guanosine, 8-OH-guanine, and 8-NO2-cGMP) were detected in plasma samples following the one-month intervention. No significant modulation of DNA and lipid damage markers was documented among groups, apart from an effect of time for DNA strand breaks and some markers of lipid peroxidation. In conclusion, the consumption of coffee and cocoa-based confectionery containing coffee was apparently not able to affect oxidative stress markers. More studies are encouraged to better explain the findings obtained and to understand the impact of different dosages of these products on specific target groups.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research