Open Accessγ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction of Bacillus subtilis A-5 and the Complete Genome Analysis
Author(s) -
Naling Bai,
Yu He,
Hanlin Zhang,
Xianqing Zheng,
Rong Zhou,
Yang Li,
Shuangxi Li,
Weiguang Lv
Publication year - 2022
Publication title -
international journal of environmental research and public health/international journal of environmental research and public health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.747
H-Index - 113
eISSN - 1661-7827
pISSN - 1660-4601
DOI - 10.3390/ijerph19137630
Subject(s) - bacillus subtilis , surfactin , gene , biology , gene cluster , plasmid , biochemistry , genome , bacteria , genetics , microbiology and biotechnology , chemistry
Bacillus subtilis A-5 has the capabilities of high-molecular-weight γ-PGA production, antagonism to plant pathogenic fungi, and salt/alkaline tolerance. This multifunctional bacterium has great potential for enhancing soil fertility and plant security in agricultural ecosystem. The genome size of B. subtilis A-5 was 4,190,775 bp, containing 1 Chr and 2 plasmids (pA and pB) with 43.37% guanine-cytosine content and 4605 coding sequences. The γ-PGA synthase gene cluster was predicted to consist of pgsBCA and factor ( pgsE ). The γ-PGA-degrading enzymes were mainly pgdS, GGT, and cwlO. Nine gene clusters producing secondary metabolite substances, namely, four unknown function gene clusters and five antibiotic synthesis gene clusters (surfactin, fengycin, bacillibactin, subtilosin_A, and bacilysin), were predicted in the genome of B. subtilis A-5 using antiSMASH. In addition, B. subtilis A-5 contained genes related to carbohydrate and protein decomposition, proline synthesis, pyruvate kinase, and stress-resistant proteins. This affords significant insights into the survival and application of B. subtilis A-5 in adverse agricultural environmental conditions.