Colon Cancer Early Detection by some Serum's Antigenic, Enzymatic and Free- serum's DNA indicators

This research include (104) patient's blood serum diagnosed with colon cancer proved by colonoscopy and histopathology. Blood samples had been selected consecutively over the period March 2013 to April 2014 from patients treated in Oncology and Nuclear Medicine hospital, AlJumhory hospital/Mosul and azadi Teaching Hospital/Kirkuk. All cases and controls were aged (2185–years). One hundred normal blood donor individuals had been used as controls. Antigenic tumor markers which include carcino-embryonic antigen (CEA) and Carbohydrate antigen 19-9 (CA 19-9) had been measured in blood's serum from 104 patients with colon cancer. Results indicate a significant elevation (p<0.05) in levels of CEA and C19-9 only in patients with advanced stage C and metastatic stage D compared with control. Beside biochemical enzymatic tumor markers that included Cyclooxygenase, Arginase showed significantly increase (p<0.05) of enzymatic activity for colon cancer patients with early stage (A) compared with controls. While the serum alkaline sphingomyelinase level was significantly decreased (p<0.05) in colon cancer patients in stage A compared with controls and this decrease was independent of Dukes’ stage, thus strengthening the hypothesized validity of these assays to be used as serum test for the early detection of colonic neoplasia. The results of this research also revealed a significant increase (p<0.05) in the serum's free DNA in all stages of colon cancer patient's compared with control grope for this it was considered as a good indicator of early detection of colon cancer.