Isolation and Identification of Microorganisms from Patients with Halitosis

Twenty-eight patients suffering from oral halitosis were subjected to this study (14 males and 14 females), their ages range between 18 to 65 years were diagnosed clinically. Ninety-five samples were collected by sterile paper points (size 50) or sterile cotton swab and transported in thioglycolate broth and cultured on blood agar in aerobic or anaerobic conditions for (48-72 hours), the identification of the bacteria was carried out using morphological and cultural characteristics, biochemical tests and antibiotics susceptibility tests. The results showed that 13 different bacterial species isolated in this study ((Bacteriodes spp. (14 isolate), Viridans Streptococci (10 isolates), Peptostreptococci spp. (9 isolates), Actinomyces spp. (6 isolates), Porphyromonas spp. (4 isolates), Fusobacterium spp. (4 isolates), Veillonella spp. (4 isolates), Non.coagulase Staphylococcus (3 isolates) Prevotella spp (one isolate), Propinobacterium spp. (one isolate) Tetragenococci spp. (one isolate), Eubacterium spp. (one isolate), and Staphylococcus aureus (one isolate). The conclusion is the dominance of different genera and species of anaerobic bactria in cases of halitosis.