Molecular Identification of Fungi Myceliopthora verrucosa which Producing Laccase Enzyme
Author(s) -
Mohammad Ibrahim Khalil,
Nadeem A. Ramadan,
Riyadh K. Albarhawi,
Joan M. Kelly
Publication year - 2014
Publication title -
mağallaẗ ʻulūm al-rāfidayn
Language(s) - English
Resource type - Journals
eISSN - 2664-2786
pISSN - 1608-9391
DOI - 10.33899/rjs.2014.86110
Subject(s) - laccase , polymerase chain reaction , enzyme , agarose , biology , fungus , dna , identification (biology) , biochemistry , chemistry , microbiology and biotechnology , botany , gene
Polymerase chain reaction (PCR) of ITS region was used to identify the fungus isolated from plastic garbage which can produce laccase enzyme. DNA bands were purified from agarose gel and then sequenced and after entering these nucleotides to the data base it was found that the DNA band belongs to the genus Myceliopthora verrucosa in 100% دمحم ميهاربإ ليلخ نورخآو 68 matching. This method is a very sensitive method for the identification of species and strains of fungi as well as its simplicity in comparing between fungi species.
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