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In this study, the Photodynamic Effect (PDE) of Hematoporphyrin derivative (HpD) combined with irradiation from low power laser on Candida albicans was investigated. Four isolates of C. albicans were diagnosed in seven samples collected from oral cavity of adults with dental caries. Laser irradiation used in this study has been emitting from Diode laser at a wavelength equal to 630 nm (red region of the spectrum), while the output power was approximately 10mW as a continuous wave. The exposure time of irradiation was between (30-240) seconds, irradiation area was (0.125) cm. C. albicans were exposed to irradiation after its exposure to several concentrations of HpD these concentrations were varied from (16 -128) μg/ml. The results clearly showed the Photodynamic effect in killing C. albicans which was photosensitized by HpD in combination with laser irradiation. There was no determined effect on yeast cells viability neither by the using HpD concentrations nor irradiation doses separately. It could be concluded that PDE of HpD by low power laser irradiation at wavelength 630 nm on Candida albicans was a powerful technique in growth inhibition of that pathogenic yeast In vitro.

Photodynamic Effect on Photosensitized Candida albicans with Hematoporphyrin Derivative Using Low Power Diode Laser In Vitro