Determination of Catechol Using Flow Injection -Electrochemical Detection with Banana Paste Electrode: Application to Adrenaline Determination | Zendy

Yousra M. Al. Shaker | Zendy; Amer T. Al-Taee | Zendy; Wadala A. Bashir | Zendy

Open Access

Determination of Catechol Using Flow Injection -Electrochemical Detection with Banana Paste Electrode: Application to Adrenaline Determination

Author(s) -

Yousra M. Al. Shaker,

Amer T. Al-Taee,

Wadala A. Bashir

Publication year - 2010

Publication title -

mağallaẗ ʻulūm al-rāfidayn

Language(s) - English

Resource type - Journals

eISSN - 2664-2786

pISSN - 1608-9391

DOI - 10.33899/rjs.2010.36862

Subject(s) - catechol , chemistry , electrode , electrochemistry , working electrode , reference electrode , flow injection analysis , inorganic chemistry , electroanalytical method , nuclear chemistry , detection limit , potentiometric titration , chromatography , organic chemistry

This work involves indirect determination of catechol compounds using flow-injection electrochemical detector. The detector based on the three-electrodes system, banana paste [which contained the polyphenyl oxidase (PPO) enzyme] as working electrode, auxiliary electrode Pt wire and silver/silver chloride, saturated potassium chloride Ag/AgCl .sat.KCl as reference electrode. The PPO enzyme in banana paste motivates the oxidation of the two hydroxyl groups in catechol to the o-quinone which is then reduced at(-0.65)V back to catechol. The reduction current was recorded and it is proportional to concentration of catechol. The technique has been applied to adrenaline hormone. ــــ

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