Isolation of infectious bronchitis virus from broiler | Zendy

M. Y. Al-Attar | Zendy; F. F. AL-Haseerchy | Zendy

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Isolation of infectious bronchitis virus from broiler

Author(s) -

M. Y. Al-Attar,

F. F. AL-Haseerchy

Publication year - 2007

Publication title -

al-mağallaẗ al-ʻirāqiyyaẗ li-l-ʻulūm al-bayṭariyyaẗ/iraqi journal of veterinary sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.391

H-Index - 9

eISSN - 2071-1255

pISSN - 1607-3894

DOI - 10.33899/ijvs.2007.5636

Subject(s) - virus , inoculation , infectious bronchitis virus , virology , biology , antiserum , microbiology and biotechnology , yolk sac , embryo , antibody , immunology

Infectious bronchitis virus (IBV) was isolated from broilers chicks. The virus isolation done by samples inoculation in chicken egg embryos via allontoic cavity route which showed sensitivity for virus isolation resulting in stunting and rounding of inoculated embryos, as well as thickening of chorioallantoic membrane (CAM). Cultivation of the isolated virus was carried out in the yolk sac and embryo vein .The virus was grown in chicken kidney cells (CKC) cultures resulting in cytopathic effect ( CPE ) after 24 h. post inoculation including swelling and accumulation of cells which increased in 2 nd & 3 rd day post inoculation. The diagnosis of isolated virus was carried out by Agar gel

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