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In this study, the effects of autotransplantation and laser irradiation on the healing of experimentally induced non-union fractures in the femoral bone were investigated clinically and radiographically in 12 dogs. The fractures were left without fixation for 40 days and over, and following the occurrence of non-union state the fractures were treated at 3 different schedules in the experimental animals, which were divided into three groups, each containing 4 dogs: Group 1 (Control): The non-union fractured femoral bones were treated by the routine surgical procedures using intramedullary fixation achieved with Steinmann intramedullary pins. Group 2 (Intramedullary fixation with autotransplantation of bone marrow): Following the surgical treatment of non-union fractured femoral bone and their fixation by the Steinmann intramedullary pins (by the same procedure applied in group 1), 4-6 ml of fresh bone marrow was implanted at the fracture site. The bone marrow was aspirated freshly and aseptically from marrow cavity of the opposite femur from the same dog. Group 3 (Autotransplantation of bone marrow with He: Ne I.R. Laser irradiation): In addition to the procedures applied in group 2, the surgical site was exposed to laser irradiation type He : Ne I.R. with a total dose of 2.7 J/cm2 for 12 irradiation sessions. Each daily irradiation session was for a period of 5 min for 3 successive days, followed by one-day rest. The concluded results were: 1- Autotransplantation of fresh bone marrow at the site of the treated non-union fractures has a role in accelerating or improving the healing process, by promoting excessive callus formation, whereby the fracture line disappeared at the end of the 4 th post-treatment week. In comparison, the callus in group 1 was characterized by its poor quality and the fracture line disappeared at the end of the 5 th post-treatment week. 2- The use of laser irradiation with autotransplantation of fresh bone marrow, greatly improved the healing process by increasing the periosteal reaction and callus formation, and lead to disappearance of the fracture line at the end of the 3 rd post-treatment week. Furthermore, this technique improved the functional use of the affected limb in comparisons with groups 1 and 2. 3- The laser irradiation made the remodeling stage shorter ( finished within about 3 months) in comparison with groups 1 and 2, in which it continued for more than 3 months.

The effect of autotransplantation of bone marrow with laser irradiation on the healing of non-union fractures in the femoral bone of dogs