Study the ability of some gram positive bacillus species isolated from powdered infant milk to produce toxin

Forty samples of different types of powdered infant milk had been collected including (celia 1-2 , biomil 1-2 , lery 1-2 , dialak 1-2 , albadee 2 ,nido 2 , kikos 2 , nictalia 1-2 , cerelak ). Some of the milk cans were opened directly at the beginning of test while others had been opened three days or more before testing. The results showed that Bacillus species were isolated and identified at different rates as Bacillus cereus has the highest rate (12.5%) then B.circulance and B. laterosporous at (5%) for each of them respectively, while B.megaterium was isolated at a rate of (2.5%). The ability of the isolated Bacillus species to produce enterotoxin was detected by the use of Rapid and delayed permeability test for the detection of the heat stable enterotoxin and the results were recorded by the appearance of blue non necrotic lesions after injection of the rabbit with the cell free culture supernated (CFCS) and Lysate supernatant (LS) of the isolated Bacillus species, and for the detection of heat labile enterotoxin, the paw oedema test was also used , the increase in thickness of fore and hind arms appeared. م ةلوزعسلا ما رك ةغبرل ةبجهسلا ةيهرعلا ايرتكبلا عاهنا ضعب ةردق ةسا رد ن بيمح . .. 100 The ability of the Bacillus species to produce the enzyme lecithinase and lyse red blood cells were also studied and the results revealed that only Bacillus cereus produce the enzyme lecithinase. in addition all the isolates showed positive hemolytic activity. :ةصلاخلا عسج ةسا رجلا ةجم ؿلاخ ؼت 04 تشسزت ةفمتخم عاؾنابو ففجسلا ؿافطلاا بيمح ؽم ةشيع ( celia 1-2 ، biomil 1-2 ، lery 1-2 ، ؾلاايد 1-2 ، عيجبلا 1-2 ، وجين 2 ، زؾكيك 2 ، ايلاتكن 12 ، )ؾلايخس ىمع ىزم خخلاا ؼدقلاو ثحبلا ءا خجا ءاشثا بيمحلا بمع ؽم اًسدق تحتفُ جقو ايحتف 3 ـا خك ةغبرل ةبجؾسلا ةيؾرعلا ايختكبلا عاؾنا ضعب صيخذتو ؿدع ؼت جقو .خثكا وا ـايا Bacillus sp . ايختكب فا جئاتشلا تخيعا ثيح ةفمتخم بدشبو Bacillus cereus تلدع تغمب ثيح ةبدن ىمعاب (12.5%) ايتمت ؼث B.circulans و B.laterosporus ةبدشب ( 5% تلدع ؽيح يف يلاؾتلا ىمع اسيشم لكل ) B.megaterium ( ةبدشب 2.5% ) . ايختكب ؽم ةلودعسلا عاؾنلاا ةرجق ؽع فذكلا ةسا رجلا ؿلاخ ؼت اسك Bacillus جاتنا ىمع او عيخدلا ةيذافشلا رابتخا ـاجختساب ػلذو ةيؾعسلا تانافيحلا بنرلاا جمجل خخأتسل Rapid and delayed permeability test of rabbit skin ةرا خحلاب تباثلا فافيحلا ؽع فذكمل heat stable enterotoxins جعب ةنخقتم خيغ ءاقرز قطاشم ؽيؾكتب ةبجؾسلا ةجيتشلا تغحؾل ثيح ( ػب بنرلاا ؽقح 0.1 ؼس ) 3 ؽم CFCS) ) Supernatant Culture Free Cell ػلاو Lysate Supernatant (LS) عاؾنلأ Bacillus فا خئفلا فكا رـؾت رابتخا ءا خجا ؼت اسك .ةلودعسلا ( paw oedema test ) ةرا خحلاب ةفلاتلا ةيؾعسلا تانافيحلا ؽع فذكمل ػلذو heat labile enterotoxins .فا خئفمل ةيفمخلاو ةيماملاا فكلاا ػسس ةدايدب ةبجؾسلا ةجيتشلا تخيع ثيح و ايختكب عاؾنا ةرجق ؽع فذكلا اًزيا ؼت جق Bacillus ؼيدنا جاتنا ىمع ةلودعسلا ايختكب تخيعا ثيح , ـجلا لمحت ىمع ايترجق ؽع فذكلا ػلحكو ديشيثدملا Bacillus cereus احيل ةبلاس ةجيتن تظعأف ةلودعسلا عاؾنلاا ةيقب اما ديشيثدملا ؼيدنا جاتنا ىمع ةرجقلا طقف عا اسك .رابتخلاا ـجلا لمحت رابتخلا ةبجؾم ةجيتن تلادعلا عيسج تخي . :ةمدقسلا خصاشعلا ؼىا وئاؾتحلا ةيلاع ةيئاحغ ةسيق ػمسي ثيح فادنلإل لماك ءاحغ بيمحلا جعي تا رجيىؾبراكلاو تاشيتوخبلا ىمع يؾتحي ؾيف ؾسشمل ةيروخزلا ,تاشيماتيفلاو فداعسلاو فؾىجلاو ظسو خبتعي ؾيف ةيئاحغلا خصاشعلا هحى ىمع بيمحلا ءاؾتحا ببدبو ًا سئلام ًا ؽم خيثك ؾسشل اًجج