Spectrophotometric determination of Salbutamol sulphate by coupling with diazotized 2,4-dinitroaniline

A quick, convenient and sensitive method has been developed for the determination of microgram amounts of salbutamol sulphate in its pure form and pharmaceutical preparations. The method is based on the coupling reaction of the drug with diazotized 2,4 dinitroaniline reagent in an alkaline medium to produce an intense blue coloured Water soluble and stable azo dye which exhibits a maximum absorption at 558 nm. Beer's law was obeyed over the concentration range 0.2 6 μg/ml with a molar absorptivity of 9.33×10 L.mol.cm and Sandell's sensitivity index of 0.0061 g/cm. The limit of detection is 0.0089 μg/ml while the Spectrophotometric determination of Salbutamol sulphate by coupling with ... 55 limit of quantitation is 0.029 μg/ml. The method shows high accuracy (average recovery 99.75%) and precision (relative standard division (RSD) is less than 2.2%.The suggested procedure was applied for determination of salbutamol sulphate without any interference from common pharmaceutical excipients. The proposed method is successfully compared with the official method. Introduction Salbutamol is RS-[4-[2-(tert-butylamino)-1-hydroxyethyl]-2(hydroxymethyl)phenol] sulphate (Scheme1). Salbutamol belongs to the family of medicines known as adrenergic bronchodilators. Adrenergic bronchodilators are medicines that are breathed in through the mouth to open up the bronchial tubes (air passages) in the lungs. The relieve cough, wheezing, shotness of breath, and troubled breathing by increasing the flow of air through the bronchial tubes.