Determination of Glucose Concentration By Rotation of Polarization Plane of He-Ne Laser

In this research, the optical activity of glucose solution had been studied through the studying of influence of this solution in rotation of polarization plane of laser He-Ne light, the different concentrations of Determination of Glucose Concentration By Rotation of Polarization Plane of ... 70 glucose solution were selected for analyzing the intensity of polarized laser light within the range o o 90 0 and by increment (10), which is transmitted during the samples of distilled water (as a standard sample) and these different concentrations of glucose solution by using analyzer. Then the relationship between values of analyzer angles and intensity values was plotted. At steady intensity for all curves the analyzer angle for distilled water and each one of concentrations has determined, then all concentrations were plotted as a function of these angles and by fitting the result curve by using MATLAB Package the polynomial of first order which represents the relationship between the rotational angle and concentrations had inquired. In other hand, the distribution in (3-D) of intensity of He-Ne laser spot which is transmitted during the samples of distilled water and the different concentrations of glucose solution was studied through taking the image for each case by using digital camera. The difference in distribution of intensity in spot of laser for each case had determined that due to different in solution concentrations. 1Introduction When a beam of plane-polarized light is directed along the optic axis of quartz, it is found that the plane of polarization turns steadily about the direction of the beam, as shown as in Fig (1), and emerges vibrating in some other plane than that at which it entered. The amount of this rotation is found experimentally to depend upon the distance traveled in the medium and upon the wavelength of the light. The former fact shows that the action occurs within the medium and not at the surface. This phenomenon of the rotation of the plane of vibration is frequently called optical activity, and many substance are now known to exhibit the effect. Some of these are cinnabar, sodium chlorate, turpentine, sugar crystals, sugar in solution, and sulfate of strychnine.[3] Some quartz crystals and sugar solution rotate the plane of vibration to the right and some to the left. Substance which rotate to the Fig (1): Rotation of the plane of vibration in an optically