Evaluation Of Lipid Peroxidation And The Level Of Some Antioxidants In People Under General Anesthesia

This research includes study of the effects of general anesthesia on lipid peroxide formation and some antioxidant system in serum of patients with appendix surgery. Forty seven patients under general anesthesia with age range of 15-45 years living in Mosul city were selected. The level of serum malondialdehyde (MDA), reduced form of glutathione (GSH), superoxide dismutase (SOD), uric acid, albumin and ceruloplasmin were measured. The results showed a significant increased in MDA level (as an index of lipid peroxidation) in patients under pentothal anesthesia in comparison with preoperative samples. On the other hand, patients under general anesthesia had decreased GSH concentration, SOD activity, uric acid, albumin and ceruloplasmin concentration when compared with preoperative samples. Evaluation Of Lipid Peroxidation And The Level Of Some Antioxidants In ... 2 INTRODUCTION Despite improvements in surgical techniques, anaesthesia, is associated with oxidative stress. Oxidative stress is a disturbance in the balance between the formation of oxidizing species (reactive oxygen species and other radicals) and their effective removal by protective antioxidants (AOX). Overwhelming radicals generated in the bloodstream and tissues can induce oxidative damage, representing by lipid peroxidation, to the cell membranes, lipoproteins, proteins, and deoxyribonucleic acid. The peroxidation of membrane phospholipids generates malondialdehyde (MDA) an end product of lipid peroxidation and a marker of oxidative stress produced during general anesthesia in dogs. On the other hand, recent interest has been focused on the role of AOX to prevent such injury produced by oxidative stress. Glutathione, superoxide dismutase, uric acid, albumin, and Ceruloplasmin possesses antioxidant activity, were used in the present study. On this basis, measurements of parameters for oxidative stress are a good technique to express the degree of cell damage. Accordingly, the aim of this study was to measure the degree of lipid peroxidation, expressed by plasma MDA, which is a reliable index for oxidative stress in vivo, and antioxidative system activity changes in patients with appendectomy under general anesthesia by Pentothal and flothin. SUBJECTS AND METHODS The Experiment Forty seven patients aged 15-45 years, 21 men and 26 women, undergoing general anesthesia of appendix surgery (appendectomy), were consecutively enrolled in a prospective observational setting. Anaesthesia was induced with pentothal 5mg/kg, which acts as hypnotic in addition to anesthetic agent. Sodium thiopental, better known as Sodium Pentothalis, is a rapid-onset short-acting barbiturate general anaesthetic. Following intravenous injection, the drug rapidly reaches the brain and causes unconsciousness within 30–45 seconds, and in about 5– 10 minutes the concentration is low enough in the brain such that consciousness returns. For this reason, thiopental is not used to maintain anesthesia in surgical procedures. Instead, anesthesia is usually maintained with an inhaled anesthetic gas, flothin 1-2% which continue to the end of operation, usually for 35-50 min. (the time consuming for appendectomy procedures). This study was conducted in Al-Salam Teaching Hospital, Mosul City, and approved by the local institutional review board, and all of the participants provided written informed consent. None of the patients had a history of previous diabetics, chronic inflammatory conditions, hepatic