Effect of the aqueous extract and some proteinous compounds from Aleo vera leaves on Blood serum glucose and cholesterol levels in normal mice

Two proteinous compounds (I and II) had been isolated by gel filtration chromatography of the precipitate produced by full saturation of the aqueous extract of the Aloe vera. The apparent molecular weights by gel filtration chromatography of the isolated compounds (I and II) were in the range of (63095,19952) Dalton respectively. Effect of the aqueous extract and some proteinous compounds ... 45 The effect of the aqueous extract and the proteinous fractions on glucose and cholesterol levels in normal mice were investigated. Glucose level was lowered by intraperitoneally administration of the concentrated aqueous extract and the low molecular weight proteinous compound (II) by (41.77% and 36.71%) respectively in normal mice. Furthermore, there was non significant reduction in the cholesterol levels by intraperitoneally administration of high molecular weight proteinous compound (Peak I) by (19.98%) in normal mice. Also there was non significant reduction in the glucose levels by orally administration of (40 mg/10 ml) of low molecular weight proteinous compound (Peak II) by (15.56%). Introduction Aloe is a succulent plant, mostly found in East and south Africa that has been used medicinally for centuries. It belongs to the family (Liliaceae) Botanically known as Aloe barbadenesis, Mill. The medicinal properties of various parts(Leaf, Aloe gel) which are rich in minerals (magnesium, calicium, chromium, copper, iron, ...etc), enzymes, amino acids, vitamins as antioxidant. Aloe vera plays an important role in many medical properties as a traditional medicine in many diseases such as Diabetes mellitus, Burns, Ulcerative colitis, Psoriasis, Wound healing. Despite the fact that more than (1200) plants are used around the world in the empirical control of Diabetes mellitus, most of them have not been pharmacologically and chemically investigated. Diabetes mellitus is a chronic disease characterized by elevated blood glucose levels and disturbance in carbohydrates, fats and protein metabolism. Milder hyperglycemia, if present for many years, increase the risk of cardiovascular disease which can manifest as a heart attack, congestive heart failure and kidney failure. The plant contains small chains of polysaccharides, which may regulate blood sugar levels may reduce inflammatory and balance insulin production in the pancrease. In heart disease patients, Aloe vera leaf added to the diet, reduced total serum cholesterol, serum triglycerides, fasting and post prandial blood sugar levels. The objective of this investigation was to evaluate the hypoglycemic activity of the aqueous extract and porteinous fractions of aloe vera in experimental mice.