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The single-celled trichomes of  Arabidopsis thaliana  have long served as an elegant model for elucidating the mechanisms of cell differentiation and morphogenesis due to their unique growth patterns. To identify new components in the genetic network that governs trichome development, we carried out exhaustive screens for additional Arabidopsis mutants with altered trichome morphology. Here, we report one mutant,  aberrantly branched trichome1-1  ( abt1-1 ), with a reduced trichome branching phenotype. After positional cloning, a point mutation in the  SPIKE1  ( SPK1 ) gene was identified in  abt1-1 . Further genetic complementation experiments confirmed that  abt1-1  is a new allele of  SPK1 , so  abt1-1  was renamed as  spk1-7  according to the literatures.  spk1-7  and two other  spk1  mutant alleles, covering a spectrum of phenotypic severity, highlighted the distinct responses of developmental programs to different  SPK1  mutations. Although null  spk1  mutants are lethal and show defects in plant stature, trichome and epidermal pavement cell development, only trichome branching is affected in  spk1-7 . Surprisingly, we found that  SPK1  is involved in the positioning of nuclei in the trichome cells. Lastly, through double mutant analysis, we found the coordinated regulation of trichome branching between  SPK1  and two other trichome branching regulators,  ANGUSTIFOLIA  ( AN ) and  ZWICHEL  ( ZWI ).  SPK1  might serve for the precise positioning of trichome nuclei, while  AN  and  ZWI  contribute to the formation of branch points through governing the cMTs dynamics. In summary, this study presented a fully viable new mutant allele of  SPK1  and shed new light on the regulation of trichome branching and other developmental processes by  SPK1 .

A New Allele of the SPIKE1 Locus Reveals Distinct Regulation of Trichome and Pavement Cell Development and Plant Growth