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Although many members encoding different ammonium- and nitrate-transporters (AMTs, NRTs) were identified and functionally characterized from several plant species, little is known about molecular components forNH    4    +- andNO    3    -acquisition/transport in tobacco, which is often used as a plant model for biological studies besides its agricultural and industrial interest. We reported here the first molecular identification in tobacco ( Nicotiana tabacum ) of nine  AMTs  and four  NRTs , which are respectively divided into four ( AMT1/2/3/4 ) and two ( NRT1/2 ) clusters and whose functionalities were preliminarily evidenced by heterologous functional-complementation in yeast or Arabidopsis. Tissue-specific transcriptional profiling by qPCR revealed that  NtAMT1.1 / NRT1.1  mRNA occurred widely in leaves, flower organs and roots; only  NtAMT1.1/1.3/2.1NRT1.2/2.2  were strongly transcribed in the aged leaves, implying their dominant roles in N-remobilization from source/senescent tissues. N-dependent expression analysis showed a marked upregulation of  NtAMT1.1  in the roots by N-starvation and resupply with N includingNH    4    +, suggesting a predominant action of NtAMT1.1 inNH    4    +uptake/transport whenever required. The obvious leaf-expression of other  NtAMTs  e.g.,  AMT1.2  responsive to N indicates a major place, where they may play transport roles associated with plant N-status and (NH    4    +-)N movement within aerial-parts. The preferentially root-specific transcription of  NtNRT1.1/1.2/2.1  responsive to N argues their importance for rootNO    3    -uptake and even sensing in root systems. Moreover, of all  NtAMT s/ NRTs , only  NtAMT1.1 / NRT1.1/1.2  showed their root-expression alteration in a typical diurnal-oscillation pattern, reflecting likely their significant roles in root N-acquisition regulated by internal N-demand influenced by diurnal-dependent assimilation and translocation of carbohydrates from shoots. This suggestion could be supported at least in part by sucrose- and MSX-affected transcriptional-regulation of  NtNRT1.1/1.2 . Thus, present data provide valuable molecular bases for the existence of  AMTs/NRT s in tobacco, promoting a deeper understanding of their biological functions.

frontiers in plant science

Coding-Sequence Identification and Transcriptional Profiling of Nine AMTs and Four NRTs From Tobacco Revealed Their Differential Regulation by Developmental Stages, Nitrogen Nutrition, and Photoperiod