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Clip-domain serine proteases (CLIPs) play crucial roles in insect development and innate immunity. In this study, we identified a CLIP gene (designated  Ls CLIP3) from the cigarette beetle  Lasioderma serricorne .  Ls CLIP3 contains a 1,773-bp open reading frame (ORF) encoding a 390-amino-acid protein and shows a conserved clip domain and a trypsin-like serine protease domain. Phylogenetic analysis indicated that  Ls CLIP3 was orthologous to the CLIP-B subfamily.  Ls CLIP3 was prominently expressed in larva, pupa, and early adult stages. In larval tissues, it was highly expressed in the integument and fat body. The expression of  Ls CLIP3 was induced by 20-hydroxyecdysone. A similar induction was also found by peptidoglycans from  Escherichia coli  and  Staphylococcus aureus . RNA interference (RNAi)-mediated silencing of  Ls CLIP3 disrupted larval–pupal molting and specifically reduced the expression of genes in 20-hydroxyecdysone synthesis and signaling pathway. The chitin amounts of  Ls CLIP3 RNAi larvae were greatly decreased, and expressions of six chitin metabolic-related genes were significantly reduced. Knockdown of  Ls CLIP3 increased larval sensitivity to Gram-negative and Gram-positive bacteria. There was significantly decreased expression of four antimicrobial peptide (AMP) genes. The results suggest that  Ls CLIP3 is an important component of the larva to pupa molt and for the immunity of  L. serricorne .

Clip-Domain Serine Protease Gene (LsCLIP3) Is Essential for Larval–Pupal Molting and Immunity in Lasioderma serricorne