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Our previous study found that  LeDnaJ07  RNAi decreased  Lentinula edodes  resistance to heat stress and  Trichoderma atroviride  infection. In this study, the structure and function of the  LeDnaJ07  gene was analyzed by gene cloning and overexpression in  L. edodes  stress-sensitive strain YS55 via the  Agrobacterium -mediated transformation method. Transformants were confirmed by qRT-PCR, fluorescence observation and Southern blotting. Overexpression of  LeDnaJ07  in YS55 not only enhanced  L. edodes  mycelial resistance to heat stress but also facilitated mycelial growth. In the presence of heat stress, the intracellular IAA content showed a significant increase in the two  LeDnaJ07  overexpression strains but only a slight change in the YS55 wild type strain. Moreover, the interaction between LeDnaJ07 and LetrpE was demonstrated via Y2H and BiFC assays. These results suggested that LeDnaJ07 may be involved in regulating IAA biosynthesis and the resistance of  L. edodes  to heat stresses via interacting with LetrpE.

Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of Lentinula edodes and Regulates IAA Biosynthesis by Interacting LetrpE