English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The locus of heat resistance (LHR) confers extreme heat resistance in  Escherichia coli . This study explored the role of the LHR in heat and pressure resistance of  E. coli , as well as its relationship with protein folding and aggregation  in vivo . The role of LHR was investigated in  E. coli  MG1655 and the pressure resistant  E. coli  LMM1010 expressing an  ibpA-yfp  fusion protein to visualize inclusion bodies by fluorescence microscopy. The expression of proteins by the LHR was determined by proteomic analysis; inclusion bodies of untreated and treated cells were also analyzed by proteomics, and by fluorescent microscopy. In total, 11 proteins of LHR were expressed: sHSP20, ClpK GI , sHSP, YdfX1 and YdfX2, HdeD, KefB, Trx, PsiE, DegP, and a hypothetical protein. The proteomic analysis of inclusion bodies revealed a differential abundance of proteins related to oxidative stress in strains carrying the LHR. The LHR reduced the presence of inclusion bodies after heat or pressure treatment, indicating that proteins expressed by the LHR prevent protein aggregation, or disaggregate proteins. This phenotype of the LHR was also conferred by expression of a fragment containing only sHSP20, ClpK GI , and sHSP. The LHR and the fragment encoding only sHSP20, ClpK GI , and sHSP also enhanced pressure resistance in  E. coli  MG1655 but had no effect on pressure resistance of  E. coli  LMM1010. In conclusion, the LHR confers pressure resistance to some strains of  E. coli , and reduces protein aggregation. Pressure and heat resistance are also dependent on additional LHR-encoded functions.

Heat and Pressure Resistance in Escherichia coli Relates to Protein Folding and Aggregation