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Phosphoglycerate kinase (Pgk), catalyzing the reversible conversions between glycerate-1.3-2P and glycerate-3P, plays an important role in carbohydrate metabolism. Here, we show that a Pgk-deficient mutant (NΔ pgk ) of  Xanthomonas axonopodis  pv.  glycines  ( Xag ) could grow in medium with glucose, galactose, fructose, mannose, or sucrose, as the sole carbon source, suggesting that  Xag  may employ Entner-Doudoroff (ED) and pentose phosphate pathway (PPP), but not glycolysis, to catabolize glucose. NΔ pgk  could not utilize pyruvate, suggesting that Pgk might be essential for gluconeogenesis. Mutation in  pgk  led to a reduction of extracellular polysaccharide (EPS) biosynthesis, cell motility, and intracellular ATP. As a result, the virulence of NΔ pgk  was significantly compromised in soybean. NΔ pgk  could be fully complemented by the wild-type  pgk , but not by  clp  (encoding Crp-like protein). qRT-PCR analyses demonstrated that  pgk  is regulated by the HrpG/HrpX cascade, but not by Clp. These results suggest that Pgk is involved in carbohydrate utilization, EPS biosynthesis, and cell motility of  Xag  independent of Clp.

frontiers in microbiology

Phosphoglycerate Kinase Is Involved in Carbohydrate Utilization, Extracellular Polysaccharide Biosynthesis, and Cell Motility of Xanthomonas axonopodis pv. glycines Independent of Clp