The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells | Zendy

Eric Daniel Ávila-Calderón | Zendy; Jorge Erick Otero-Olarra | Zendy; Leopoldo FloresRomo | Zendy; Humberto Peralta | Zendy; Ma. Guadalupe AguileraArreola | Zendy; María Rosario Morales-García | Zendy; Juana CalderónAmador | Zendy; Olín Medina-Chávez | Zendy; Luis DonisMaturano | Zendy; María del Socorro Ruiz-Palma | Zendy; Araceli Contreras-Rodríguez | Zendy

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The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

Author(s) -

Eric Daniel Ávila-Calderón,

Jorge Erick Otero-Olarra,

Leopoldo FloresRomo,

Humberto Peralta,

Ma. Guadalupe AguileraArreola,

María Rosario Morales-García,

Juana CalderónAmador,

Olín Medina-Chávez,

Luis DonisMaturano,

María del Socorro Ruiz-Palma,

Araceli Contreras-Rodríguez

Publication year - 2018

Publication title -

frontiers in microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.701

H-Index - 135

ISSN - 1664-302X

DOI - 10.3389/fmicb.2018.02765

Subject(s) - aeromonas hydrophila , microbiology and biotechnology , vesicle , bacterial outer membrane , virulence , biology , extracellular , hemolysin , hemolysis , bacteria , chemistry , biochemistry , escherichia coli , membrane , immunology , gene , genetics

Gram-negative bacteria release outer membrane vesicles (OMVs) into the extracellular environment. OMVs have been studied extensively in bacterial pathogens, however, information related with the composition of Aeromonas hydrophila OMVs is missing. In this study we analyzed the composition of purified OMVs from A. hydrophila ATCC ® 7966 TM by proteomics. Also we studied the effect of OMVs on human peripheral blood mononuclear cells (PBMCs). Vesicles were grown in agar plates and then purified through ultracentrifugation steps. Purified vesicles showed an average diameter of 90–170 nm. Moreover, 211 unique proteins were found in OMVs from A. hydrophila ; some of them are well-known as virulence factors such as: haemolysin Ahh1, RtxA toxin, extracellular lipase, HcpA protein, among others. OMVs from A. hydrophila ATCC ® 7966 TM induced lymphocyte activation and apoptosis in monocytes, as well as over-expression of pro-inflammatory cytokines. This work contributed to the knowledge of the composition of the vesicles of A. hydrophila ATCC ® 7966 TM and their interaction with the host cell.

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