Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital | Zendy

Chendi Zhu | Zendy; Veranja Liyanapathirana | Zendy; Carmen Li | Zendy; Vasanthi Pinto | Zendy; Mamie Hui | Zendy; Norman Lo | Zendy; K. T. Wong | Zendy; Nilanthi Dissanayake | Zendy; Margaret Ip | Zendy

Open Access

Characterizing Mobilized Virulence Factors and Multidrug Resistance Genes in Carbapenemase-Producing Klebsiella pneumoniae in a Sri Lankan Hospital

Author(s) -

Chendi Zhu,

Veranja Liyanapathirana,

Carmen Li,

Vasanthi Pinto,

Mamie Hui,

Norman Lo,

K. T. Wong,

Nilanthi Dissanayake,

Margaret Ip

Publication year - 2018

Publication title -

frontiers in microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.701

H-Index - 135

ISSN - 1664-302X

DOI - 10.3389/fmicb.2018.02044

Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , plasmid , virulence , biology , multiple drug resistance , quinolone , mobile genetic elements , enterobacteriaceae , klebsiella , antibiotic resistance , gene , drug resistance , antibiotics , genetics , escherichia coli

Limited data is available on the epidemiology and characteristics of carbapenem-resistant Enterobacteriaceae (CRE) and their associated plasmids or virulence determinants from Sri Lanka. Through whole genome sequencing of CREs from the intensive care units of a Sri Lankan teaching hospital, we identified a carbapenemase gene, bla OXA–181 in 10 carbapenemase-producing Klebsiella pneumoniae isolates (two strains of ST437 and eight strains of ST147) from 379 respiratory specimens. bla OXA–181 was carried in three variants of ColE-type plasmids. K. pneumoniae strains with ompK36 variants showed high minimum inhibitory concentrations to carbapenem. Furthermore, genes encoding for extended spectrum β-lactamases (ESBL), plasmid-mediated quinolone resistance (PMQR) determinants ( qnr , aac(6′)-Ib-cr , and oqxAB ) were present in all 10 strains. Amino acid substitution in chromosomal quinolone resistance-determining regions (QRDRs) gyrA (Ser83Ile) and parC (Ser80Ile) were also observed. All strains had yersiniabactin genes on mobile element ICE kp . Strict infection control practices and judicious use of antibiotics are warranted to prevent further spread of multidrug-resistant K. pneumoniae .

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