Molecular Signatures of Neuroinflammation Induced by αSynuclein Aggregates in Microglial Cells

Alpha-synuclein (αSyn Agg ) are pathological hallmarks of Parkinson's disease (PD) and other synucleinopathies that induce microglial activation and immune-mediated neurotoxicity, but the molecular mechanisms of αSyn Agg -induced immune activation are poorly defined. We performed quantitative proteomics by mass spectrometry coupled with PCR, immunohistochemical and functional validations studies to define the molecular characteristics of alpha synuclein mediated microglial activation. In mouse microglia, αSyn Agg induced robust pro-inflammatory activation (increased expression of 864 genes including Irg1, Ifit1 , and Pyhin ) and increased nuclear proteins involved in RNA synthesis, splicing, and anti-viral defense mechanisms. Conversely, αSyn Agg decreased expression several proteins (including Cdc123, Sod1, and Grn), which were predominantly cytosolic and involved in metabolic, proteasomal and lysosomal mechanisms. Pathway analyses and confirmatory in vitro studies suggested that αSyn Agg partly mediates its effects via Stat3 activation. As predicted by our proteomic findings, we verified that αSyn Agg induces mitochondrial dysfunction in microglia. Twenty-six proteins differentially expressed by αSyn Agg were also identified as PD risk genes in genome-wide association studies (upregulated: Brd2, Clk1, Siglec1; down-regulated: Memo1, Arhgap18, Fyn, and Pgrn/ Grn ). We validated progranulin (PGRN) as a lysosomal PD-associated protein that is downregulated by αSyn Agg in microglia in-vivo and is expressed by microglia in post-mortem PD brain, congruent with our in vitro findings. Conclusion: Together, proteomics approach both reveals novel molecular insights into αSyn-mediated neuroinflammation in PD and other synucleinopathies.