Callus Induction and In Vitro Plant Regeneration of Indian Maize Inbreds by Embryo Culture. | Zendy

Rohini Kiran Kunta . | Zendy; K.B.L. Jyothi . | Zendy; Shabana Begum | Zendy

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Callus Induction and In Vitro Plant Regeneration of Indian Maize Inbreds by Embryo Culture.

Author(s) -

Rohini Kiran Kunta .,

K.B.L. Jyothi .,

Shabana Begum

Publication year - 2012

Publication title -

journal of current pharma research

Language(s) - English

Resource type - Journals

eISSN - 2230-7842

pISSN - 2230-7834

DOI - 10.33786/jcpr.2012.v02i02.008

Subject(s) - callus , embryo culture , biology , regeneration (biology) , micropropagation , embryo , tissue culture , in vitro , botany , microbiology and biotechnology , agronomy , genetics , embryogenesis

Regeneration ability and callus induction of three Indian maize inbred lines HKI335 and HKI1105 and LM5 were evaluated. Immature embryos were used as explants. Genotype, medium type of auxin and their concentrations influenced callus induction MS medium supplemented with different concentrations of 2, 4 D (0.5 – 2 mg/L) were used for callus induction. MS media supplemented with 2mg/L of 2, 4D has shown highest percentage of callus induction 80%. Among the three genotypes tested HKI335 and HKI1105 both have shown higher regeneration percentage.

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