Study of Heat Shock Protein: In Housefly.

Insects and other animals, including humans, produce heat-shock proteins in response to extremely high temperatures. Hsp is vital in withstanding stress conditions which insects normally thrive, the aim of our work was to study Hsps and find out the molecular weight of Hsps in Musca domestica. Houseflies were collected. The samples prepared were loaded on SDS gel along with protein molecular weight marker and study of Hsps was done by using silver staining technique. The test samples which was exposed to heat shock at 42oC & 45oC were compared with control which was prepared by growing larvae at optimum temperature i.e. 26°C-28°C. It was found that survival rate was not decrease along with increase in temperature. Thus, Hsp’s play very important role when Hsp’s expressed through stress conditions. Hsps was increased when cells were exposed to elevated temperature. A band of approximately 70 kDa was seen to be expressed in all the Hsps as against control sample.