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A Reverse phase method has been developed for the quantitative estimation of Atenolol, Metoprolol Tartarate and Propanolol Hydrochloride in Drug Substance.. The Quantification was carried out using RP stainless steel column ODS C18 250 x 4.6 x 5 μ L1 packing in Isocratic mode with mobile phase containing 0.03 M potassium buffer; Acetonitrile and Methanol in the ratio of 58 :21:21 and at pH3.5 ± 0.05. Flow rate was 1.0 ml/minute and the detection wavelength was set at 227 nm. The linearity was found to be in the range of 8-12 μg/ml for Atenolol and Metoprolol Tartarate and Propanolol Hydrochloride 4-6 μg/ml .The proposed method was found to be simple, precise, accurate, reproducible for the Estimation of the three drugs in presence of each other.

journal of current pharma research

Development and validation of a simultaneous HPLC method for Quantification of Atenolol, Metoprolol Tartarate and Propanolol Hydrochloride in Drug substance.