Open AccessA Simple RP-HPLC Method for Estimation of Triclabendazole and Ivermectin in a Pharmaceutical Suspension Dosage Form.
Author(s) -
K . Nischal,
B . Somshekar,
P M . Abhilekha,
K C . Sharadamma,
P. Radhakrishna
Publication year - 2011
Publication title -
journal of current pharma research
Language(s) - English
Resource type - Journals
eISSN - 2230-7842
pISSN - 2230-7834
DOI - 10.33786/jcpr.2011.v01i04.003
Subject(s) - triclabendazole , ivermectin , chromatography , suspension (topology) , dosage form , simple (philosophy) , chemistry , mathematics , medicine , veterinary medicine , philosophy , epistemology , homotopy , helminths , pure mathematics , immunology , fasciola hepatica
A simple method has been developed for the estimation of triclabendazole and ivermectin in a pharmaceutical suspension dosage form present in 50:1 ratio respectively, by reversed-phase High-Performance Liquid Chromatography (HPLC). The isocratic LC analysis was performed on Vydac, C-18, (250 X 4.6 mm, 5 μ) using mobile phase comprising of acetonitrile, methanol and water at a flow rate of 1.5 ml/minute. Quantification was carried out by using UV detector at 254 nm and the run time was 15 minutes. Linearity was found to be 0.060.14 and 0.03-0.05 mg/ml with a correlation co-efficient, r= 0.998 and 0.999 for triclabendazole and ivermectin respectively. The percentage recovery values were found to be within the range of 98-102. The analytical method has been successively applied to pharmaceutical formulation and was validated according to International Conference on Harmonization (ICH) guidelines.
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