Development of fingerprints for an ayurvedic formulation Ajmodadi churna, via piperine estimation by UV-Spectrophotometry

A simple and reproducible UV-spectrophotometric method for the quantitative determination of piperine in Ajmodadi churna (AJC) were developed and validated in the present work. The parameters linearity, precision , accuracy, limit of detection ,limit of quantification and standard error were studies according to international conference on harmonization guidelines. In this present study a new, simple, rapid, sensitive, precise and economic spectrophotometric method in ultraviolet region has been developed for the determination of piperine in market and laboratory herbal formulation of Ajmodadi churna. Which were procured and purchased respectively from the local market and they were evaluated as per Indian Pharmacopoeia and WHO guidelines. Piperine has the maximum wavelength at 342.5 nm and hence the UV spectrophotometric method was performed at 342.5 nm. The samples were prepared in methanol and methods obey Beers law in concentration ranges employed for evaluation. The content of piperine in ayurvedic formulation was determined. The result of the present study revealed that all six batches of Ajmodadi churna were found in close proximity. The result of analysis has been validated statistically and recovery studies confirmed the accuracy of the proposed method. Hence the proposed method can be used for the reliable quantification of Piperine in crude drug and its herbal formulation.