miR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line

miRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids containing pri-miRNA sequences for these two miRNAs and introduced them into the A1235 glioblastoma cell line. Clones containing increased expression of processed miR-7 and miR-34a were obtained. The proliferation and sensitivity to alkylation agent of transfected cells were similar to those of control cells. Our results indicate that an increase in miR-7 and miR34 expression alone in A1235 glioblastoma cells is not sufficient to change their proliferation or sensitivity to the influence of alkylating agents. 1 Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia 2 Division of Molecular Biology, Rudjer Boskovic Institute, Zagreb, Croatia Corresponding author: Maja Matulic Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102, 10000 Zagreb, Croatia Tel: +385 1 4606 278 Fax: +385 1 4606 286 e-mail: mmatulic@biol.pmf.hr Submitted: July, 2020 Accepted: September, 2020