Enumeration of sandy sediment bacteria: search for optimal protocol | Zendy

Epstein Ss | Zendy; J Rossel | Zendy

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Enumeration of sandy sediment bacteria: search for optimal protocol

Author(s) -

Epstein Ss,

J Rossel

Publication year - 1995

Publication title -

marine ecology progress series

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.151

H-Index - 188

eISSN - 1616-1599

pISSN - 0171-8630

DOI - 10.3354/meps117289

Subject(s) - enumeration , sediment , bacteria , protocol (science) , biology , environmental science , mathematics , combinatorics , paleontology , medicine , alternative medicine , pathology

We examined and compared a variety of existing protocols for enumeration of bacteria from marine sandy sediments. The focus was on how to dislodge bacteria from sediment particles; a commercial blender, an ultrasonic cleaner, and an ultrasonic cell disrupter were tested. The ultrasonic cell disrupter was found to be the most efficient device for bacterial dislodgment. With a , sediment bacteria enumeration (use of surfactants, thoroughness of sample washing, bacterial staining and counterstaining, etc.), striving for additional ways of improvement. The result was a protocol that, in our study, gave bacterial counts superior to those obtained by any other protocol currently in use KEY WORDS: Bacteria . Benthos Enumeration . Fluorescence Ultrasonic treatment

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