Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis | Zendy

SooUng Lee | Zendy; Sun Huh | Zendy

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Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis

Author(s) -

SooUng Lee,

Sun Huh

Publication year - 2004

Publication title -

korean journal of parasitology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.555

H-Index - 42

eISSN - 1738-0006

pISSN - 0023-4001

DOI - 10.3347/kjp.2004.42.3.145

Subject(s) - clonorchis sinensis , internal transcribed spacer , biology , mitochondrial dna , nuclear dna , sequence analysis , ribosomal dna , cytochrome c oxidase , nadh dehydrogenase , genetics , ribosomal rna , gene , phylogenetics , zoology , mitochondrion , helminths

We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.

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