Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai | Zendy

SooUng Lee | Zendy; Sun Huh | Zendy; WoonMok Sohn | Zendy; Jong-Yil Chai | Zendy

Open Access

Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai

Author(s) -

SooUng Lee,

Sun Huh,

WoonMok Sohn,

Jong-Yil Chai

Publication year - 2004

Publication title -

korean journal of parasitology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.555

H-Index - 42

eISSN - 1738-0006

pISSN - 0023-4001

DOI - 10.3347/kjp.2004.42.3.129

Subject(s) - mitochondrial dna , cytochrome c oxidase , ribosomal rna , biology , protein subunit , ribosomal dna , nadh dehydrogenase , microbiology and biotechnology , genetics , computational biology , mitochondrion , phylogenetic tree , gene

We compared the DNA sequences of the genus Metagonimus: M. yokogawai, M. takahashii, and M. miyatai. We obtained 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome c oxidase subunit I (mtCOI) fragments from the adult worms by PCR, that were cloned and sequenced. Phylogenetic relationships inferred from the nucleotide sequences of the 28S D1 rDNA and mtCOI gene. M. takahashii and M. yokogawai are placed in the same clade supported by DNA sequence and phylogenic tree analysis in 28S D1 rDNA and mtCOI gene region. The above findings tell us that M. takahashii is closer to M. yokogawai than to M. miyatai genetically. This phylogenetic data also support the nomination of M. miyatai as a separate species.

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