Immunodiagnosis of clonorchiasis using a recombinant antigen | Zendy

TaiSoon Yong | Zendy; Hye Jin Yang | Zendy; SoonJung Park | Zendy; Y K Kim | Zendy; D H Lee | Zendy; S M Lee | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Immunodiagnosis of clonorchiasis using a recombinant antigen

Author(s) -

TaiSoon Yong,

Hye Jin Yang,

SoonJung Park,

Y K Kim,

D H Lee,

S M Lee

Publication year - 1998

Publication title -

korean journal of parasitology

Language(s) - English

Resource type - Journals

eISSN - 1738-0006

pISSN - 0023-4001

DOI - 10.3347/kjp.1998.36.3.183

Subject(s) - clonorchis sinensis , clonorchiasis , recombinant dna , biology , microbiology and biotechnology , antigen , complementary dna , escherichia coli , clone (java method) , gene , western blot , cdna library , virology , biochemistry , genetics , immunology , helminths

A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a beta-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research