Cloning and sequencing of p33 in a Korean isolate of Theileria sergenti
Author(s) -
SangWook Kang,
EunJin Choi,
Chang Hee Kweon
Publication year - 1997
Publication title -
korean journal of parasitology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.555
H-Index - 42
eISSN - 1738-0006
pISSN - 0023-4001
DOI - 10.3347/kjp.1997.35.2.105
Subject(s) - open reading frame , homology (biology) , cloning (programming) , gene , nucleic acid sequence , biology , genetics , peptide sequence , sequence (biology) , sequence analysis , nucleotide , amino acid , microbiology and biotechnology , virology , computer science , programming language
The gene encoding the 33 kDa piroplasm surface protein of Theileria sergenti isolated in Korea was cloned and the nucleotide sequence was determined by dideoxy chain termination method. The cloned gene corresponds to 869 bp encoding an open reading frame 283 amino acids. Comparison of the sequence between Korean and Japanese isolates showed 99.4% homology rate in the nucleotide sequence and 98.9% homology rate in the amino acid sequence.
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