Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to Acanthamoeba polyphaga | Zendy

Hyun-Hee Kong | Zendy; JungHyun Park | Zendy; Dong-Il Chung | Zendy

Open Access

Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to Acanthamoeba polyphaga

Author(s) -

Hyun-Hee Kong,

JungHyun Park,

Dong-Il Chung

Publication year - 1995

Publication title -

korean journal of parasitology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.555

H-Index - 42

eISSN - 1738-0006

pISSN - 0023-4001

DOI - 10.3347/kjp.1995.33.4.331

Subject(s) - biology , isozyme , microbiology and biotechnology , mitochondrial dna , aminopeptidase , dna , restriction fragment length polymorphism , genetics , biochemistry , enzyme , gene , polymerase chain reaction , leucine , amino acid

Interstrain polymorphisms of isoenzyme profiles and mitochondrial (Mt) DNA fingerprints were observed among seven strains of Acanthamoeba isolated from different sources and morphologically assigned to A. polyphaga. Mt DNA fingerprints by eight restriction endonucleases (Bgl II, Sca I, Cla I, EcoR I, Xba I, Kpn I, Sal I, and Sst I) revealed considerable interstrain polymorphisms. Isoenzyme profiles revealed considerable interstrain polymorphisms for acid phosphatase, lactate dehydrogenase, and glucose-6-phosphate dehydrogenase while those for glucose phosphate isomerase, leucine aminopeptidase, and malate dehydrogenase showed similarity. Despite of the interstrain polymorphisms, the isoenzyme profiles and Mt DNA fingerprints of the strain Ap were found to be identical with those of the strain Jones. Mt DNA fingerprinting was found to be highly applicable for the strain identification, characterization, and differentiation.

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