The maintenance of free-living amoebae by cryopreservation | Zendy

S A Seo | Zendy; TaiSoon Yong | Zendy; KyoungBin Im | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The maintenance of free-living amoebae by cryopreservation

Author(s) -

S A Seo,

TaiSoon Yong,

KyoungBin Im

Publication year - 1992

Publication title -

korean journal of parasitology

Language(s) - English

Resource type - Journals

eISSN - 1738-0006

pISSN - 0023-4001

DOI - 10.3347/kjp.1992.30.2.151

Subject(s) - cryopreservation , liquid nitrogen , glycerol , biology , andrology , chemistry , medicine , biochemistry , fishery , embryo , organic chemistry

We have successfully cryopreserved free-living amoebae in order to maintain them feasibly under the conditions in our laboratory. The viability of trophozoites was higher when frozen by slow cooling (overall 0.7 degree C/min) than by fast cooling (overall 1.3 degrees C/min). Glycerol and dimethylsulfoxide at the final concentration of 7.5% each was used for cryopreservation of free-living amoebae trophozoites. The survival rate was 2-39% after storage in the liquid nitrogen for 60 days. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research