Open AccessPharmacokinetics of Glutathione and Its Metabolites in Normal Subjects
Author(s) -
SaeYong Hong,
HyoWook Gil,
JongOh Yang,
EunYoung Lee,
Hyung-Kee Kim,
Soohyun Kim,
YoungHo Chung,
Soon-Jung Hwang,
ZeeWon Lee
Publication year - 2005
Publication title -
journal of korean medical science/journal of korean medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.743
H-Index - 66
eISSN - 1598-6357
pISSN - 1011-8934
DOI - 10.3346/jkms.2005.20.5.721
Subject(s) - glutathione , paraquat , cysteine , cystine , metabolite , chemistry , reactive oxygen species , pharmacology , methionine , pharmacokinetics , biochemistry , medicine , amino acid , enzyme
To determine the loading and maintenance dosage of glutathione (GSH) for patients suffering from reactive oxygen species (ROS) injury such as acute paraquat intoxication, a kinetic study of reduced GSH was performed in synchrony with that of cysteine (Cys), cystine (Cys2), and methionine (Met). Human subject's porticipitation was voluntary. The effective dose of Cys, Cys2, and Met against ROS in fibroblast cells generated by paraquat was assessed using laser scanning confocal microscopy. Both Cys and Met suppressed ROS in a dose-dependent manner at concentrations of 1-1,000 microM; the concentration required to suppress ROS by 50% was 10 microM for Cys and 50 microM for Met. Using metabolite kinetics with the assumption that Cys and Met are the metabolites of GSH, expected concentrations of Cys and Met of above 20 and 50 microM were estimated when GSH was administered at 50 mg/kg body weights every 205.4 min for Cys and 427.4 min for Met.